Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC TAE-1 25mg | 1414469-59-2 | 25 MG
TAE-1 is a small-molecule inhibitor of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) that also inhibits amyloid-β fibril formation and aggregation. It is used in biochemical and cellular research into Alzheimer's disease, cholinesterase function, and amyloid-β aggregation.
- Potent inhibitor of AChE and BuChE.
- Inhibits amyloid-β fibril formation and aggregation.
- Molecular formula C39H51I3N6O9 and molecular weight 1128.57.
- Typical supplied purity about 96.17%.
- Available in small laboratory pack sizes (for example, 25 mg) for research use.
Apexbio Technology LLC Annexin V-APC/PI Apoptosis Kit 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (APC) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes Additionally this kit can be paired with the nuclear dye propidium iodide (PI) PI specifically binds to double-stranded DNA emitting red fluorescence upon binding but it cannot normally permeate intact cell membranes However PI can penetrate compromised cellular membranes in late-stage apoptotic and dead cells staining nuclei Therefore combined staining with Annexin V-APC and PI allows
Apexbio Technology LLC Annexin V-APC Reagent 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) Under normal conditions PS is located on the inner side of the plasma membrane lipid bilayer however during early apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane becoming exposed to the extracellular environment Annexin V has a high affinity for PS Fluorescent (APC)-labeled Annexin V serves as a fluorescent probe enabling detection of apoptotic cells using flow cytometry or fluorescence microscopy The staining procedure takes only 15-30 minutes to complete Use of this reagent requires additional purchase of 10X Binding Buffer (Catalog no K2284) To further characterize different apoptosis stages Annexin V can be combined with nuclear dyes such as 7-AAD DAPI PI or SYTOX Green These nuclear dyes specifically bind double-stranded DNA generating fluorescence Normally they cannot penetrate intac
Cayman Chemical TrIs Buffer ConcentrAT10X 10mL
A thioredoxin inhibitor inhibits Trx activity in ES-2 cells at 20 UM cytotoxic to ES-2 cells (IC50 1.6 UM) induces ferroptosis in ES-2 cells at 5 UM an effect that can be blocked by DFO Trolox or ferrostatin-1 decreases tumor volume in a 4T1 murine mammary carcinoma model using immunocompetent but not immunodeficient mice at 50 mg/kg twice per week
ILEX LIFE SCIENCES LLC EDTA P10-15100 100ML
PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 0.02% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 100G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 100G
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Apexbio Technology LLC MES sodium salt 71119-23-8 1g
MES sodium salt (CAS 71119-23-8) is a buffering agent designed to maintain stable pH conditions in the range of 5 5 7 0 supporting physiological experimental environments MES sodium salt does not have a direct cellular or molecular target and is not a small-molecule inhibitor agonist or antagonist Its key role is pH stabilization in various biological and biochemical assays including enzymatic activity assays receptor pathway investigations and isolation of cellular organelles or biomolecules Experimental models include plant cell culture systems and physiological in vitro assays Based on these properties MES sodium salt holds research potential in applications such as plant cell culture controlled metabolic pathway studies and signal transduction research requiring consistent pH conditions
Biotium Fixation Permeabilization Other Buffers Buffers Accessories and Background Reducers Fixation Permeabilization and Other Buffers Chemical Reagents 50/PK
PBS (phosphate-buffered saline) buffer powder packet for preparing 2 L of 1X PBS buffer Box includes 50 packets
Biotium Fixation Permeabilization Other Buffers DNA Gel Stains and Accessories DNA Gel Stains Chemical Reagents 50/PK
TAE (Tris-acetate-EDTA) buffer powder packet for preparing 1 L of 1X TAE buffer for nucleic acid gel electrophoresis Box includes 50 packets
eMolecules TERT-BUTYL 3-(HYDROXYMETHYL)-1,4-DIAZEPANE-1-CARBOXYLATE HCL | 1179360-20-3 | MFCD23702017 | 1g
AstaTech | TERT-BUTYL 3-(HYDROXYMETHYL)-1,4-DIAZEPANE-1-CARBOXYLATE HCL | 1g | 412394943 | I11907 | 95.000 | 1179360-20-3 | MFCD23702017 | 266.770 | C11H23ClN2O3
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