Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
CHEMSCIENCE Brass "A" for Sn, Pb, Cu, Zn
• This product is supplied in five lot of 500 g each of known concentration ( % concentration availiable upon request.)
• Detailed purity information, along with the Safety Data Sheet (SDS) and Technical Data Sheet (TDS), will be provided upon purchase.
Kd Medical Inc TE 1X, PH 8.0, 500 ML
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. One 500ml bottle.
Biotang Inc Lysis Buffer(His-tag protein purification from E.Coli and insect cells), 500 ml
Lysis Buffer(His-tag protein purification from E.Coli and insect cells), 500 ml.
Boston Bioproducts Inc 10mM Tris-HCl pH 7.5 1L
Tris-HCl Buffer (10 mM pH 7 5) - 1L (For Research Use and Further Manufacturing Only)
Medchemexpress LLC Tris buffered saline | 10 TABLETS
Tris buffered saline is supplied as ready-to-dissolve tablets for preparing Tris/NaCl buffer solutions commonly used in molecular biology and protein applications. The tablet format simplifies buffer preparation and storage while delivering a stable pH environment for assays and electrophoresis.
- Ready-to-use tablet format for convenient buffer preparation.
- One tablet dissolves in 15 mL deionized water to yield 0.05 M Tris and 0.15 M sodium chloride.
- pH approximately 7.6 at 25 °C.
- White to off-white solid tablets for easy visual quality check.
- Suitable for DNA/RNA extraction, PCR, and protein electrophoresis.
- Compact packaging reduces storage space and eases transport.
Biotang Inc TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml
TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml.
Bioworld TT - SDS Running Buffer (Anode) (10X), 500 mL
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.9. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Bioworld TT - SDS Running Buffer (Cathode) (10X), 500 mL
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.3. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Sigma Aldrich Fine Chemicals Biosciences ACES >=99.0% (titration) | 7365-82-4 | MFCD00008030 | 25G
ACES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 182.2 | 7365-82-4 | MFCD00008030 | 25G
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 25g
HEPES | Purity: 99.5% | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 25g
Sigma Aldrich Fine Chemicals Biosciences HEPES >=99.5% (titration) | 7365-45-9 | MFCD00006158 | 5KG
HEPES >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 5KG