Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Thermo Scientific Production Chemicals and Services 50MM TRIS 10 MM EDTA 1.65M AM
NC3588257 50MM TRIS 10 MM EDTA 1.65M AM
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTA 5ML
NC3588252 50 MM TRIS-HCL 10 MM EDTA 5ML
Cayman Chemical Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium II dichloride complex, Purity: ≥95%, CAS: 36309-88-3, 25 mg
A luminescent probe (absorption λmax: 455 nm, luminescence λmax: 613 nm) widely used for detection and quantitation of oxygen; strongly reduced by molecular oxygen as a result of dynamic quenching; thus oxygen detection can be based on either measurement of intensity or decay time
Biotang Inc HEPES Lysis Buffer with Triton (2X), 125 ml
HEPES Lysis Buffer with Triton (2X), 125 ml.
TARGETMOL CHEMICALS INC P62-mediated mitophagy inducer
Also available in 1 mL, 2 mg, 5 mg, 10 mg, 50 mg, 100 mg, 200 mg and bulk. Please contact Fisher for quotes. P62-mediated mitophagy inducer (PMI) is a regulator of mitophagy. Purity 99.35%
eMolecules 5704-04-1 | Tricine | Oakwood Chemical | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
Tricine | Oakwood Chemical | 5704-04-1 | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
Electron Microscopy Sciences Tris Buffer 0.2M, pH 8.0 250 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 8.0
eMolecules 279255-90-2 | tert-Butyl 5-(hydroxymethyl)-1H-indole-1-carboxylate | Combi-Blocks, Inc. | MFCD09834884 | 247.294 | C14H17NO3 | 98.000 | CC(C)(C)OC(=O)n1ccc2cc(CO)ccc12 | 1g | 603139295
tert-Butyl 5-(hydroxymethyl)-1H-indole-1-carboxylate | Combi-Blocks, Inc. | 279255-90-2 | MFCD09834884 | 247.294 | C14H17NO3 | 98.000 | CC(C)(C)OC(=O)n1ccc2cc(CO)ccc12 | 1g | 603139295
Medchemexpress LLC NVP-TAE684 | 761439-42-3 | MFCD11977634 | 99.7% | 614.2 g/mol | C30H40ClN7O3S | 10 MG
NVP-TAE684 is a potent, selective small-molecule inhibitor of anaplastic lymphoma kinase (ALK) used in biochemical and cellular research. It blocks growth of ALK-dependent and ALCL-derived cell lines with low-nanomolar IC50 values and has been applied in preclinical studies to probe ALK signaling and validate target engagement. Typically supplied as a solid for in vitro experiments.
- Potent ALK inhibition at low-nanomolar concentrations.
- Selective activity against ALK-driven cell lines.
- Suitable for biochemical and cellular assays.
- Supplied as a solid for research use.
- Characterized with reported purity and analytical data.
Alkali Scientific CellPro™ Tris-HCL Buffer,1M, pH 8.0, 1L, 12/cs
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 100G
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 100G